Angiotensin II Kit FLISA

N° du produit ABIN2871086

Ce kit ELISA Humain, Rat, Souris, Chien, Porc et Cobaye Angiotensin II est un kit ELISA Fluorometric conçu pour quantifier Humain, Rat, Souris, Chien, Porc et Cobaye Angiotensin II.

Aperçu rapide pour Angiotensin II Kit FLISA (ABIN2871086)

Antigène: Angiotensin II (Ang II)

Reactivité: Humain, Rat, Souris, Chien, Porc, Cobaye

Méthode de détection: Fluorometric

Type de méthode: Competition ELISA

Application: ELISA

Détail du produit Angiotensin II Kit FLISA

Analytical Method: Quantitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Angiotensin II (Ang II)

Autre désignation: Angiotensin II

Pathways: Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity